Nano Biosols TECHNICAL

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TECHNICAL DATA

GOLD NANOPARTICLE DATA

Particle size	%CV	Gold concentration µg /ml	OD520/ml	Particles/ml	Gold Molar concentration Mol/L
5nm	<20%	50	0.8	40 x 10¹²	0.254mM
10nm	<10%	50	0.8	4.9 x 10¹²	0.254mM
20nm	<10%	50	0.85	6.2 x 10¹¹	0.254mM
40nm	<10 %	50	0.9	7.7 x 10¹⁰	0.25mM

GOLD CONJUGATE DATA

Particle size	%CV	Gold concentration µg /ml	OD520	Particles/ml	Gold Molar concentration Mol/L
5nm	<20%	125	2	100 x 10¹²	0.6mM
10nm	<10%	125	2	12.5 x 10¹²	0.6mM
20nm	<10%	125	2	14.5 x 10¹¹	0.6mM
40nm	<10 %	125	2	17.1 x 10¹⁰	0.6mM

PROTEIN PARTICLE RATIO (approximate)

Particle size	Protein A	Streptavidin	Antibody molecules *
5nm	2	2	1-2
10nm	11-15	15-18	10- 11
20nm	35-40	35-40	25-30
40nm	120-130	130-140	90-100

Based on affinity purified goat anti species antibodies .