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TECHNICAL DATA

GOLD NANOPARTICLE DATA
Particle  size %CV Gold concentration
µg /ml
OD520/ml Particles/ml Gold Molar concentration
Mol/L
5nm
 
<20%        50      0.8 40 x 1012     0.254mM
10nm
 
<10%          50      0.8 4.9 x 1012     0.254mM
20nm
 
<10%         50      0.85 6.2 x 1011     0.254mM
40nm
 
<10 %        50      0.9 7.7 x 1010     0.25mM


GOLD CONJUGATE DATA
Particle  size %CV Gold concentration
µg /ml
OD520 Particles/ml Gold Molar concentration
Mol/L
5nm
 
<20%      125       2 100 x 1012     0.6mM
10nm
 
<10%      125       2 12.5 x 1012     0.6mM
20nm
 
<10%      125       2 14.5 x 1011     0.6mM
40nm
 
<10 %      125       2 17.1 x 1010      0.6mM


PROTEIN PARTICLE RATIO (approximate)
 
Particle size Protein A Streptavidin Antibody molecules *
5nm
 
        2         2           1-2
10nm
 
       11-15     15-18           10- 11
20nm
 
      35-40    35-40           25-30
40nm
 
     120-130   130-140           90-100